The council, which vets candidates for general elections, wed out 634 hopefuls who had registered to run for the February 26 elections, Hossein-Ali Amiri said on Tuesday.
He added that 37 candidates would be vying for seats in the 88-member Assembly at Tehran Province constituency.
Amiri said the disqualified candidates will have two days to appeal with the Guardian Council. The council will examine their requests during three days.
The next elections for the 10th Majlis (parliament) and the 5th Assembly of Experts will take place simultaneously on February 26.
The high-ranking body of the Assembly of Experts elects and oversees the activities of the Leader of the Islamic Revolution, and is in a position of authority to either appoint or dismiss the Leader.
Members of the Assembly are directly elected to office by people for an eight-year term. The body holds meetings twice a year to review major national issues and every other year to appoint a new chairman.
Ayatollah Mohammad Yazdi was appointed the new chairman of the Assembly in March last year after the passing away of its former chairman, Ayatollah Mohammad-Reza Mahdavi Kani.